蒙古马血液蛋白(酶)多态性研究

研究分别对乌珠穆沁马、乌审马、锡尼河马和巴尔虎马4个蒙古马类群共计221匹马的运铁蛋白（Tf）、前白蛋白（Pr）、血清酯酶（Es）、白蛋白（ALb）、碳酸酐酶（CA）、α1-B糖蛋白（A1B）、6-磷酸葡萄糖脱氢酶（PGD）、VD结合蛋白（GC）8个蛋白座位进行检测。群体遗传变异指标计算结果表明,4个类群蒙古马8个蛋白（酶）座位的平均杂合度为：锡尼河马0.3783,巴尔虎马0.3666,乌珠穆沁马0.3296,乌审马0.3056。根据基因频率计算Nei氏遗传距离进行分析。结果表明,巴尔虎马和锡尼河马之间的遗传距离最近,它们与乌珠穆沁马、乌审马的遗传距离相对较远。     

动物腹腔手术后粘连的成因及防制概述

就术后粘连发生的诱因、发生机理进行了阐述,并结合动物粘连模型的试验研究结果及临床研究结果,对预防腹腔手术后粘连的药物和方法进行了综述。     

高丹草对波本杂羔羊的饲喂效果比较

试验选用16只波本杂F1代羔羊,按体重、性别、日龄配对分成2组,在基础饲料相同的情况下,试验组饲喂高丹草(乐食),对照组饲喂皇竹草。通过30天对比饲养试验,结果表明:试验组羔羊的平均日增重(113 g)略优于对照组(109 g),但差异不显著(P>0.05)。从而认为,高丹草饲养羔羊的效果与皇竹草相近。     

联合国探雷犬使用指南

(IMAS09.40,2003-01-01第1次出版)前言国际人道主义排雷程序标准是于1996年7月在丹麦的一个专题研讨会上提出的,会上提出了排除各种地雷的标准,并通过研讨修订后颁布了标准。至同年年底,在丹麦通过的标准最后发展成由联合国领导工作组颁布的国际人道主义排雷操作标准。此标准由     

甜菜碱对长吻(鱼危)鱼主要消化器官蛋白酶的影响

用单因子试验设计原理,将平均体长为(6±0.5)cm,平均体质量为5g/尾的长吻鱼危鱼种300尾随机分为10个试验组,在基础配方相同的饲料中分别添加0.1%、0.3%、0.5%和0.7%的甜菜碱进行养殖对比试验,测定肠道及肝胰脏中蛋白酶活性的变化,并与对照组进行对比。试验结果表明,饲料中添加甜菜碱能提高长吻鱼危鱼肠道和肝胰脏中的蛋白酶活性。肠道中蛋白酶活性以0.5%的添加量最为明显,添加量为0.3%和0.7%的次之,0.1%的添加量效果最差;肝胰脏蛋白酶活性以0.7%的添加量最为明显,添加量为0.3%和0.5%的次之,0.1%的添加量效果最差。     

2005年神农架马陆大发生原因及防治措施

马陆学名Julus bortersis属多足纲目科,分布于世界各国。国内各地都有分布,特别是温室和森林履盖率高,腐质层厚的地方均有发生。1发生危害特点2005年马陆在神农架松柏镇城镇、郊区、森林、农田、居民的住宅区、院内,靠近森林的公路、沟边暴发成灾。发生面积1 400m2,涉及城镇居民150余户,2 000人以上,布满城镇居民室内外的每一个角落,无处不有。在森林、农田、居民住宅潮湿的地方聚集成堆,在农田、森林居民居住的院内外、室内、外墙壁上呈均匀分布,有群集爬行的特点,每1m2最高有虫800~1 000头,平均1m2有虫500头以上,使城镇居民不能在家中生…     

Modifying Myxococcus xanthus protoporphyrinogen oxidase to plant codon usage and high level of oxyfluorfen resistance in transgenic rice

Protoporphyrinogen oxidase (Protox) of Myxococcus xanthus (Mx Protox) is a 49-kDa membrane protein that catalyzes conversion of protoporphyrinogen IX (Protogen IX) into protoporphyrin IX (Proto IX). Upon heterologous expression in transgenic rice plants, Mx Protox is dually targeted into plastids and mitochondria, increasing resistance against the herbicidal Protox inhibitor oxyfluorfen. Here, we describe the chemical synthesis of the Mx Protox gene by assembling several small synthetic DNA fragments derived by ligation-PCR. Codon usage in the resulting 1416-bp gene was modified to correspond to that of the Arabidopsis Protox gene, a change that resulted in a decrease in G+C content from 71 to 49%. The modified Mx Protox gene was used to generate transgenic rice plants via Agrobacterium-mediated transformation. Integration, expression, and inheritance of the transgenes were demonstrated by Southern, Northern, and Western blot analyses. In plants transformed with the modified, low G+C-content Mx Protox gene, levels of Protox expression and enzyme activity were low compared to the levels observed for plants transformed with the native Mx Protox gene. Nonetheless, like the native gene, the modified gene conferred a high level of resistance to the herbicide oxyfluorfen in a seedling growth test.     

添加酒糟和玉米粉对花生壳微贮效果的影响

通过测定单独的花生壳原样及花生壳中添加10%的酒糟或玉米粉经过微贮后其有机物(粗蛋白、粗纤维、粗脂肪、无氮浸出物)及营养价值的变化情况,研究花生壳的微贮效果。试验结果表明:花生壳经过微贮后营养价值有所提高,原料花生壳中CP含量为9.85%,而微贮后纯花生壳中CP含量为10.99%,添加酒糟和玉米粉微贮后的花生壳中CP含量分别为12.13%、13.25%,微贮后单独的花生壳、添加酒糟和玉米粉的花生壳中CP含量和原料花生壳相比分别上升了11.57%、23.15%、34.52%,添加酒糟和玉米粉的花生壳中CP与原样CP含量相比差异显著;原料花生壳中CF含量为70.82%,而微贮后纯花生壳中CF含量为69.44%,添加酒糟和玉米粉微贮后的花生壳中CF含量分别为68.56%、67.41%,微贮后纯花生壳、添加酒糟和玉米粉的花生壳中CF含量和原料花生壳相比分别下降了1.95%、3.19%、4.82%,添加酒糟和玉米粉的花生壳CF与原样CF含量相比差异显著;在花生壳中添加玉米粉效果比添加酒糟效果好。     

扶正解毒散超微粉对人工感染传染性法氏囊病预防试验

以扶正解毒散超微粉1%、0.5%、0.25%浓度拌料给药,通过死亡率、血清中抗体滴度及增重等指标评价预防效果。结果表明:扶正解毒散超微粉高、中剂量组对抗体、脾脏代偿性增重以及平均增重效果均较突出。临床推荐剂量为0.5%浓度拌料,自由采食饮水,连用5 d。     

Enhancement of protective immune responses by oral vaccination with Saccharomyces cerevisiae expressing recombinant Actinobacillus pleuropneumoniae ApxIA or ApxIIA in mice

We previously induced protective immune response by oral immunization with yeast expressing the ApxIIA antigen. The ApxI antigen is also an important factor in the protection against Actinobacillus pleuropneumoniae serotype 5 infection; therefore, the protective immunity in mice following oral immunization with Saccharomyces cerevisiae expressing either ApxIA (group C) or ApxIIA (group D) alone or both (group E) was compared with that in two control groups (group A and B). The immunogenicity of the rApxIA antigen derived from the yeast was confirmed by a high survival rate and an ApxIA-specific IgG antibody response (p < 0.01). The highest systemic (IgG) and local (IgA) humoral immune responses to ApxIA and ApxIIA were detected in group E after the third immunization (p < 0.05). The levels of IL-1β and IL-6 after challenge with an A. pleuropneumoniae field isolate did not change significantly in the vaccinated groups. The level of TNF-α increased in a time-dependent manner in group E but was not significantly different after the challenge. After the challenge, the mice in group E had a significantly lower infectious burden and a higher level of protection than the mice in the other groups (p < 0.05). The survival rate in each group was closely correlated to the immune response and histopathological observations in the lung following the challenge. These results suggested that immunity to the ApxIA antigen is required for optimal protection.